Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Carbonic Anhydrase IX while the user-added detection antibodies bind to epitopes on the captured target protein. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The OmniKine? Human Carbonic Anhydrase IX ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Carbonic Anhydrase IX concentrations within any experimental sample including cell lysates, serum and plasma.
Not to forget, the results are presented in different formats for your ease. Let’s now explore in detail the various steps that allow you to measure your ELISA readout easily. It acts as your companion, assisting you seamlessly through the entire readout and analysis process, from initial setup to obtaining instant results. When you are ready with your ELISA plate preparation, all that remains is connecting your computer to the Absorbance 96 ELISA plate reader and launching the Absorbance 96 App, a user-friendly and freely downloadable software. In this article, we won’t delve into every aspect of the ELISA workflow instead, we will focus on the last stage – the readout specifically with the Absorbance 96 App. Nonetheless, the fundamental procedure involves four key stages: coating, blocking, detection, and readout, which you may already be familiar with. There are different types of ELISAs to choose from, depending on your research needs. What sets this apart is its innate simplicity and efficiency in detecting the presence of antigens in biological samples. The ELISA (enzyme-linked immunosorbent assay) is an indispensable and highly versatile tool in the toolbox of researchers in the fields of molecular biology, biochemistry, and diagnostics worldwide.